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anti cd40 blocking antibody  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti cd40 blocking antibody
    Anti Cd40 Blocking Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40+blocking+antibody/CD40+Antibody%2C+anti-human/10__1016_slash_j__isci__2026__116175-342-29-32
    Average 96 stars, based on 210 article reviews
    anti cd40 blocking antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Stimulation:

    Article Title: Influenza strain-specific T cell responses longitudinally post-vaccination with FluZone.
    Article Snippet: Despite seasonal vaccination strategies, influenza viruses still cause significant morbidity and mortality.. While vaccine efficacy is generally high, effectiveness varies from year-to-year and can be impacted by numerous host factors, including age, sex, obesity, and immune history.. Therefore, understanding host immune factors that promote robust vaccine responses and protection from influenza infection and disease, is important for improving vaccine design and evaluation.

    Incubation:

    Article Title: Influenza strain-specific T cell responses longitudinally post-vaccination with FluZone.
    Article Snippet: Despite seasonal vaccination strategies, influenza viruses still cause significant morbidity and mortality.. While vaccine efficacy is generally high, effectiveness varies from year-to-year and can be impacted by numerous host factors, including age, sex, obesity, and immune history.. Therefore, understanding host immune factors that promote robust vaccine responses and protection from influenza infection and disease, is important for improving vaccine design and evaluation.

    Article Title: Antiviral CD4 + T and myeloid cell responses to influenza vaccines are attenuated in older adults.
    Article Snippet: .. Subsequently, cells were incubated with 0.5μg/mL CD40 blocking antibody (Miltenyi Biotec 130-108-041) for 15 minutes at 37°C. ..

    Blocking Assay:

    Article Title: Influenza strain-specific T cell responses longitudinally post-vaccination with FluZone.
    Article Snippet: Despite seasonal vaccination strategies, influenza viruses still cause significant morbidity and mortality.. While vaccine efficacy is generally high, effectiveness varies from year-to-year and can be impacted by numerous host factors, including age, sex, obesity, and immune history.. Therefore, understanding host immune factors that promote robust vaccine responses and protection from influenza infection and disease, is important for improving vaccine design and evaluation.

    Article Title: Proviruses in CD4 + T cells reactive to autologous antigens contribute to nonsuppressible HIV-1 viremia
    Article Snippet: .. For the activation-induced marker (AIM) assay, 1ug/mL of CD40 blocking antibody (Clone HB14, Miltenyi) was supplemented into media to prevent CD154 down-regulation. .. After 24 hours of activation, cells were resuspended with 75uL of wash media and stained with 2uL of CD69 FITC (Clone FN50, BioLegend), 2uL of CD154 BV421 (Clone 24-31, BioLegend), and 2uL CD137 PE (Clone 4B4-1, BioLegend) for activation markers.

    Article Title: Limited vaccine-induced CD8+ T cell immunity in HIV-infected immunological nonresponders.
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/ mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Article Title: Limited vaccine-induced CD8 + T cell immunity in HIV-infected immunological nonresponders
    Article Snippet: Subsequently, cells were stimulated for 1 h at 37°C with costimulation (anti-CD28, BD Biosciences) and either a pool of 182 OLPs (10 μg/mL) covering the entire spike protein of SARS-CoV-2, phytohemagglutinin M (PHA-M, 10 μg/mL) as a positive control, or with an equimolar amount of DMSO as a negative control. .. In addition, cells used for the AIM assay were treated with CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) to enable surface staining of CD40L. ..

    Article Title: Proviruses in CD4 + T cells reactive to autologous antigens contribute to nonsuppressible HIV-1 viremia
    Article Snippet: .. For the activation-induced marker (AIM) assay, 1μg/ml of CD40 blocking antibody (Clone HB14, Miltenyi Biotec) was supplemented into media to prevent CD154 down-regulation. .. After 24 hours of activation, cells were spun down and resuspended with 75μl of wash media, as described above, and stained with 2μl of CD69 FITC (Clone FN50, BioLegend, cat 310904), 2μl of CD154 BV421 (Clone 24–31, BioLegend, cat 310824), and 2μl CD137 PE (Clone 4B4–1, BioLegend, cat 309804) for activation markers.

    Article Title: Antiviral CD4 + T and myeloid cell responses to influenza vaccines are attenuated in older adults.
    Article Snippet: .. Subsequently, cells were incubated with 0.5μg/mL CD40 blocking antibody (Miltenyi Biotec 130-108-041) for 15 minutes at 37°C. ..

    Article Title: Limited vaccine-induced CD8 + T cell immunity in HIV-infected immunological nonresponders
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Article Title: Limited vaccine-induced CD8+ T cell immunity in HIV-infected immunological nonresponders.
    Article Snippet: Subsequently, cells were stimulated for 1 h at 37°C with costimulation (anti-CD28, BD Biosciences) and either a pool of 182 OLPs (10 μg/mL) covering the entire spike protein of SARS-CoV-2, phytohemagglutinin M (PHA-M, 10 μg/mL) as a positive control, or with an equimolar amount of DMSO as a negative control. .. In addition, cells used for the AIM assay were treated with CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) to enable surface staining of CD40L. ..

    Activation Assay:

    Article Title: Proviruses in CD4 + T cells reactive to autologous antigens contribute to nonsuppressible HIV-1 viremia
    Article Snippet: .. For the activation-induced marker (AIM) assay, 1ug/mL of CD40 blocking antibody (Clone HB14, Miltenyi) was supplemented into media to prevent CD154 down-regulation. .. After 24 hours of activation, cells were resuspended with 75uL of wash media and stained with 2uL of CD69 FITC (Clone FN50, BioLegend), 2uL of CD154 BV421 (Clone 24-31, BioLegend), and 2uL CD137 PE (Clone 4B4-1, BioLegend) for activation markers.

    Article Title: Proviruses in CD4 + T cells reactive to autologous antigens contribute to nonsuppressible HIV-1 viremia
    Article Snippet: .. For the activation-induced marker (AIM) assay, 1μg/ml of CD40 blocking antibody (Clone HB14, Miltenyi Biotec) was supplemented into media to prevent CD154 down-regulation. .. After 24 hours of activation, cells were spun down and resuspended with 75μl of wash media, as described above, and stained with 2μl of CD69 FITC (Clone FN50, BioLegend, cat 310904), 2μl of CD154 BV421 (Clone 24–31, BioLegend, cat 310824), and 2μl CD137 PE (Clone 4B4–1, BioLegend, cat 309804) for activation markers.

    Marker:

    Article Title: Proviruses in CD4 + T cells reactive to autologous antigens contribute to nonsuppressible HIV-1 viremia
    Article Snippet: .. For the activation-induced marker (AIM) assay, 1ug/mL of CD40 blocking antibody (Clone HB14, Miltenyi) was supplemented into media to prevent CD154 down-regulation. .. After 24 hours of activation, cells were resuspended with 75uL of wash media and stained with 2uL of CD69 FITC (Clone FN50, BioLegend), 2uL of CD154 BV421 (Clone 24-31, BioLegend), and 2uL CD137 PE (Clone 4B4-1, BioLegend) for activation markers.

    Article Title: Proviruses in CD4 + T cells reactive to autologous antigens contribute to nonsuppressible HIV-1 viremia
    Article Snippet: .. For the activation-induced marker (AIM) assay, 1μg/ml of CD40 blocking antibody (Clone HB14, Miltenyi Biotec) was supplemented into media to prevent CD154 down-regulation. .. After 24 hours of activation, cells were spun down and resuspended with 75μl of wash media, as described above, and stained with 2μl of CD69 FITC (Clone FN50, BioLegend, cat 310904), 2μl of CD154 BV421 (Clone 24–31, BioLegend, cat 310824), and 2μl CD137 PE (Clone 4B4–1, BioLegend, cat 309804) for activation markers.

    Cell Culture:

    Article Title: Limited vaccine-induced CD8+ T cell immunity in HIV-infected immunological nonresponders.
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/ mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Article Title: Limited vaccine-induced CD8 + T cell immunity in HIV-infected immunological nonresponders
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Positive Control:

    Article Title: Limited vaccine-induced CD8+ T cell immunity in HIV-infected immunological nonresponders.
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/ mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Article Title: Limited vaccine-induced CD8 + T cell immunity in HIV-infected immunological nonresponders
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Negative Control:

    Article Title: Limited vaccine-induced CD8+ T cell immunity in HIV-infected immunological nonresponders.
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/ mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Article Title: Limited vaccine-induced CD8 + T cell immunity in HIV-infected immunological nonresponders
    Article Snippet: .. Cells for AIM assay were cultured in the presence of costimulation, antibodies targeting chemokine receptors, CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control). .. Cells for ICS were cultured in the presence of costimulation, antibodies targeting chemokine receptors, brefeldin A (GolgiPlug, 0.5 μL/mL, BD Biosciences) and either the spike OLP pool (1 μg/mL), PHA-M (5 μg/mL; positive control) or DMSO (5 mg/mL; negative control).

    Staining:

    Article Title: Limited vaccine-induced CD8 + T cell immunity in HIV-infected immunological nonresponders
    Article Snippet: Subsequently, cells were stimulated for 1 h at 37°C with costimulation (anti-CD28, BD Biosciences) and either a pool of 182 OLPs (10 μg/mL) covering the entire spike protein of SARS-CoV-2, phytohemagglutinin M (PHA-M, 10 μg/mL) as a positive control, or with an equimolar amount of DMSO as a negative control. .. In addition, cells used for the AIM assay were treated with CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) to enable surface staining of CD40L. ..

    Article Title: Limited vaccine-induced CD8+ T cell immunity in HIV-infected immunological nonresponders.
    Article Snippet: Subsequently, cells were stimulated for 1 h at 37°C with costimulation (anti-CD28, BD Biosciences) and either a pool of 182 OLPs (10 μg/mL) covering the entire spike protein of SARS-CoV-2, phytohemagglutinin M (PHA-M, 10 μg/mL) as a positive control, or with an equimolar amount of DMSO as a negative control. .. In addition, cells used for the AIM assay were treated with CD40 blocking antibody (0.5 μg/mL, Miltenyi Biotec) to enable surface staining of CD40L. ..



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